Center for Lignocellulose Structure and Formation

About the Center

EFRCs (Energy Frontier Research Centers)

The Center for Lignocellulose Structure and Formation (CLSF) is an Energy Frontier Research Center (EFRC) initially established in 2009 by the US Department of Energy to accelerate fundamental research and scientific breakthroughs in energy-relevant areas to meet critical energy challenges of the 21st century. EFRCs integrate the expertise of multiple leading scientific investigators to enable fundamental research of a scope and complexity that are beyond beyond the capabilities of single investigator or small-group research projects and to provide an environment that encourages high-risk, high-reward research that would not be done otherwise. The CLSF received renewed funding in 2014 based both on our achievements to date and the quality of our proposal for future research. More information about the Department of Energy's EFRC program can be found at the DOE EFRC website.

Our mission: CLSF is working to dramatically increase our fundamental knowledge of the physical structure of biopolymers in plant cell walls to enable fresh insights and perspectives into the formation and structure of these highly useful, energy-rich, renewable biomaterials.

Understanding the fundamentals of cell wall structure and assembly may open new frontiers in bioenergy and biomaterials technologies based on cell wall engineering. Cellulose microfibrils and plant cell walls are far beyond anything that human technology can replicate today. We cannot make synthetic cellulose with properties anywhere close to what nature makes. Much of our research aims to elucidate how living plant cells assemble simple sugars into complex, hierarchically organized, polymeric nanocomposites that are beyond existing human abilities to manufacture.


The EFRCs are called to be synergistic: our strength is the collaborations between researchers with different backgrounds: biologists, physicists, chemists, engineers, computional scientists. Penn State University is the lead institution with partners at North Carolina State University, University of Rhode Island, University of Virginia School of Medicine, Massachsetts Institute of Technology, University of Texas at El Paso, Stony Brook University, University of Chicago, and Oak Ridge National Laboratory each of which contributes special expertise to the Center. Please visit our People page to learn more about each of our Senior Investigators' research interests.

Lignocellulose Focus

Lignocellulose is the major structural material of plant bodies and constitutes the enormously important biorenewable resource used to make building materials, paper, textiles and many polymer derivatives. At the nanoscale, lignocellulose is a highly versatile composite of three complex biopolymers, namely, crystalline nm-scale fibrils of cellulose which are linked together by less-ordered polysaccharides (such as xylans) and embedded in lignin, a complex and heterogeneous phenolic macromolecule.

Despite its huge economic importance, many questions about how plants can convert soluble sugar into high-strength crystalline cellulose microfibrils and combine them with other polymers to make cell walls with diverse physical properties. Plants lock up the majority of their stored photosynthetically-captured solar energy in their cell walls: lignocellulose is an important biomaterial and the largest store of renewable solar energy on Earth. Honed by 500 million years of evolution and refinement, the nanoscale construction of the plant cell wall endows it with a remarkable combination of mechanical properties and resistance to enzymatic and chemical attack. Its intricate and complex structure makes the wall recalcitrant to conversion to biofuels, necessitating expensive and energy-intensive pretreatments as an early step for biofuel production. A deeper understanding of the physico-chemical interactions underlying cell wall structure and its assembly may open new frontiers in bioenergy and biomaterials technologies based on cell wall engineering. This research can offer insights into ways to synthesize complex, hierarchical biomaterials and to modulate the chemical, mechanical and other physical properties of such biomaterials.

Our Work

CLSF continues to enhance its state-of-the-art transdisciplinary research to uncover the details of plant cell wall structure and cellulose microfibril formation.  The work of our center is organized around these basic questions:

  1. Lignocellulose illustrationHow do plants synthesize para-crystalline cellulose microfibrils?
    1. What are the key structure/function relationships in plant cellulose synthases (CESAs)?
    2. What is the structure and composition of the plant cellulose synthase complex and how does it produce the cellulose microfibril?
  2. How do cellulose and other components form extensible cell walls during growth and stable, reinforced walls at maturity?
    1. How are nano- and meso-scale structure and assembly linked to growth and mechanics in primary cell walls?
    2. What are the nano- and meso-scale details of secondary cell wall structure, and how are they formed during secondary wall synthesis?

The center is comprised of a unique mix of experts in computational modeling, biochemistry, biophysics, biopolymer analytics, cell biology, genetics, molecular biology, polymer physics, synthetic chemistry, and systems biology specifically chosen to work synergisterically to tackle key questions of lignocellulose structure and formation, using both experimental and theoretical (including computational) approaches.


The fundamental knowledge and technical expertise to be developed by the Center is essential for designing novel ways to manipulate plant cell walls, an important step in unlocking the energy-rich cell wall for the next generation of sustainable biofuels and for creating new cellulosic biomaterials with diverse economic applications. Additionally, the understanding of how nature creates this most versatile of biocomposites could be used to create new composites based on different polymers.

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